The Intelligence, Surveillance, and Reconnaissance (ISR) community relies heavily on the use of overhead imagery for object detection and classification. In these applications, machine learning frameworks have been increasingly used to assist analysts in distinguishing high value targets from mundane objects quickly and effectively. In recent years, the robustness of these frameworks has come under question due to the possibility for disruption using image-based adversarial attacks, and as such, it is necessary to harden existing models against these threats. In this work, we survey a collection of three techniques to address these concerns at various stages of the image processing pipeline: external validation using Activity Based Intelligence, internal validation using Latent Space Analysis, and adversarial prevention using biologically inspired techniques. We found that biologically-inspired techniques were most effective and generalizable for mitigating adversarial attacks on overhead imagery in machine learning frameworks, with improvements as much as 34.6% over traditional augmentations, and 80.4% over a model without any augmentation-based defense.