The objective the study was to determine the seroprevalence of Toxopasma gondii in adult female llamas from two peasant communities from the province of Melgar, Puno. Blood samples were collected in 30 and 77 llamas from Huaycho and Huataywasi communities. Samples were analyzed by the indirect immunofluorescent test. The seroprevalence to T. gondii was 36.7% in Huaycho and 6.5% in Huataywasi. Both communities showed different conditions and management systems, which can explain the high differences in seroprevalence.