We report for the first time the occurrences in Brazil of Boana hobbsi, Osteocephalus deridens and Tepuihyla shushupe (Hylidae), and confirm the occurrences of Ranitomeya defleri and Ranitomeya variabilis (Dendrobatidae) referring voucher specimens obtained from an expedition to Estação Ecológica Juami-Japurá, state of Amazonas, Brazil, in February 2017. Additionally, we provide data on morphology of voucher specimens, advertisement calls, field observations and geographic distribution of these species. We also describe the external morphology of the female R. defleri, which was previously unknown to science.