Objective:This study aimed to investigate the mediating effects of eudemonic well-being (EWB) at work for the associations between psychosocial work environment and overall EWB among Japanese workers.Methods:We conducted a cross-sectional study using data from a 2018 survey. psychosocial work environment measures included: job demands, job control, and supervisor and coworker support. Path analysis using structural equation modeling and mediation analysis were applied to examine the mediating effects of work EWB.Results:A total of 834 workers were analyzed. Work EWB completely mediated the associations of job control (β=0.288, P<0.001) and coworker support with overall EWB (β=0.178, P<0.001).Conclusions:The effects of job control and coworker support associated with overall EWB were fully mediated by work EWB.