Title
Development and validation of a rapid RP-HPLC method for the determination of Racecadotril in formulation
Date Issued
01 December 2009
Access level
metadata only access
Resource Type
journal article
Author(s)
Department of Quality Control, Granules India Limited
Abstract
A simple rapid specific precise and accurate reverse phase high performance liquid chromatographic method was developed for the determination of Racecadotril (RACE) in sachet dosage forms using atorvastatin as internal standard. Ratio of the peak area of analyte to internal standard was used to calculate for quantification. A phenomenex-Luna RP-18, 5mm column having 250x4.6 mm i.d. in isocratic mode, with mobile phase containing acetonitrile: 0.05M phosphate buffer (potassium dihydrogen orthophosphate): triethylemine (80:19.95:0.05) adjusted to pH 3.95 using orthophosphoric acid. The flow rate was 1.0ml/min and effluents were monitored at 231nm. The retention times of atorastatin calcium and racecaclotril were 3.453 min and 4.210 min respectively. Linearity was observed over concentration range of 10-80 μg/ml. The recovery of racecadotril was found to be in the range of 99.6-100.5%. The proposed method was validated successfully and applied to the estimation of a racecadotril in sachet dosage forms.
Start page
1090
End page
1093
Volume
1
Issue
4
Language
English
OCDE Knowledge area
Química analítica
Subjects
Scopus EID
2-s2.0-77953439677
Source
International Journal of ChemTech Research
Sources of information:
Directorio de Producción Científica
Scopus