Title
Short report: Polymerase chain reaction for chronic Trypanosoma cruzi infection yields higher sensitivity in blood clot than buffy coat or whole blood specimens
Date Issued
01 January 2008
Access level
metadata only access
Resource Type
journal article
Author(s)
Fitzwater S.
LaFuente C.
Ferrufino L.
Bern C.
Universidad Peruana Cayetano Heredia
Johns Hopkins University
Universidad Peruana Cayetano Heredia
Johns Hopkins University
Publisher(s)
American Society of Tropical Medicine and Hygiene
Abstract
Trypanosoma cruzi polymerase chain reaction (PCR) is widely used, but sensitivity varies widely. We compared PCR using 121/122 primers targeting kinetoplast minicircle DNA in whole blood, buffy coat, and clot from Bolivian women. Sensitivity was significantly higher in clot (60.1%) than buffy coat (46.5%) or whole blood (40%). The use of clot could simplify specimen collection while improving sensitivity. Copyright © 2008 by The American Society of Tropical Medicine and Hygiene.
Start page
768
End page
770
Volume
79
Issue
5
Language
English
OCDE Knowledge area
Enfermedades infecciosas
Scopus EID
2-s2.0-55849112284
PubMed ID
Source
American Journal of Tropical Medicine and Hygiene
ISSN of the container
00029637
Sponsor(s)
National Institute of Allergy and Infectious Diseases / R21AI072093, T35AI065385.
Fogarty International Center / D43TW006581.
Sources of information:
Directorio de Producción Científica
Scopus