Title
Comparison of sand fly trapping approaches for vector surveillance of leishmania and bartonella species in ecologically distinct, endemic regions of peru
Date Issued
01 July 2021
Access level
open access
Resource Type
journal article
Author(s)
Lozano M.E.
Espada L.J.
Kosoy M.
McKee C.
Arevalo H.
Troyes M.
Stoops C.A.
Fisher M.L.
Naval Medical Research Unit South
Naval Medical Research Unit South
Naval Medical Research Unit South
Publisher(s)
Public Library of Science
Abstract
Background In Peru, the information regarding sand fly vectors of leishmaniasis and bartonellosis in the Amazon region is limited. In this study, we carried out sand fly collections in Peruvian lowland and highland jungle areas using different trap type configurations and screened them for Leishmania and Bartonella DNA. Methodology/Principal findings Phlebotomine sand flies were collected in Peruvian Amazon jungle and inter Andean regions using CDC light trap, UV and color LED traps, Mosquito Magnet trap, BG Sentinel trap, and a Shannon trap placed outside the houses. Leishmania spp. screening was performed by kDNA PCR and confirmed by a nested cytochrome B gene (cytB) PCR. Bartonella spp. screening was performed by ITS PCR and confirmed by citrate synthase gene (gltA). The PCR amplicons were sequenced to identify Leishmania and Bartonella species. UV and Blue LED traps collected the highest average number of sand flies per hour in low jungle; UV, Mosquito Magnet and Shannon traps in high jungle; and Mosquito Magnet in inter Andean region. Leishmania guyanensis in Lutzomyia carrerai carrerai and L. naiffi in Lu. hirsuta hirsuta were identified based on cytB sequencing. Bartonella spp. related to Bartonella bacilliformis in Lu. whitmani, Lu. nevesi, Lu. hirsuta hirsuta and Lu. sherlocki, and a Bartonella sp. related to Candidatus B. rondoniensis in Lu. nevesi and Lu. maranonensis were identified based on gltA gene sequencing. Conclusions/Significance UV, Blue LED, Mosquito Magnet and Shannon traps were more efficient than the BG-Sentinel, Green, and Red LED traps. This is the first report of L. naiffi and of two genotypes of Bartonella spp. related to B. bacilliformis and Candidatus B. rondoniensis infecting sand fly species from the Amazon region in Peru.
Volume
15
Issue
7
Language
English
OCDE Knowledge area
Enfermedades infecciosas
Medicina tropical
Scopus EID
2-s2.0-85111734195
PubMed ID
Source
PLoS Neglected Tropical Diseases
ISSN of the container
19352727
Sponsor(s)
GV received funding for this study from the U.S. DoD Armed Forces Health Surveillance Division (AFHSD), Global Emerging Infections Surveillance (GEIS) Branch ProMIS ID P0106_18_N6_05 for FY2018, ProMIS ID P0143_19_N6_04 for FY2019, and the Advanced Medical Development Program Proposal UID3. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank to Albino Urquia from (DIRESA-Madre de Dios); Danti Toribio, Juan Ruiz Tananta (DIRESA San Martin); Lilian Zaquinaula, Soledad Juarez, Juan Hernandez (DISA Jaen Cajamarca); and Nelson Solorzano (Caraz Hospital, Ancash) for all their support in fieldwork activities for sand fly collections. We would like to especially thank to Dr. Lynn Osikowicz (Centers for Disease Control and Prevention, Division of Vector-Borne Diseases, USA) for her suggestions. We also appreciate the support of Dr. Hirotomo Kato (Jichi Medical University, Japan) forLeishmania detection in sand flies. We would like to thank to Dr. Juan Pablo Murillo (Medicine Faculty, San Marcos University, Lima-Peru) for the valuable input and suggestions for entomological data analysis. Our thanks to NAMRU-6 departments of Parasitology (Dr. Maxy De Los Santos) and Bacteriology (Enrique Canal) who kindly provided us with positive controls ofLeishmania andBartonella, respectively. Our recognition to Mr. Roberto Fernandez (Department of Entomology NAMRU-6) for his help and recommendations for taxonomical identification of phlebotomine sand fly species. We are grateful to the Ministerio de Agricultura y Riego de Peru, Direccion General Forestal y de Fauna Silvestre for permission to conduct these studies under the auspices of Resolución Directoral No. 0306-2013-MINAGRI-DGFFS/DGEFFS.
Sources of information:
Directorio de Producción Científica
Scopus