In medicine, the diagnosis of diseases by means of image processing has had great acceptability and credibility, which is why it has been able to explore in depth the theme, taking advantage of mainly which is a non-invasive method within the clinical study. Glaucoma is considered as a group of diseases that damages the optic nerve to the point of causing blindness in the eye, with the disadvantage of in most cases do not exhibit early symptoms. Through this work, the processing biomedical images to locate the parameters considered most relevant within images obtained from the back of the eye to make the determination of the existence or not of Glaucoma, a disease that mainly affects physically the dimensions and proportions of the cup and the optical disc. It gets the value of the reason cup/disc and is additional to the characteristics of the rule ISNT, both parameters considered of great importance in the diagnosis of glaucoma. The results obtained serve as a tool to support the specialist in ophthalmology and optometry in conjunction with a presumptive diagnosis of glaucoma, this way it looks for the early detection and prevention of this disease.