The research provides a new and sustainable methodology for the synthesis of silver nanoparticles, using Eucalyptus globulus extract, this due to the fact that it presents metabolites capable of acting as a reducing potential of our silver nitrate precursor, and thus obtaining nanostructured material. This is also associated with the reuse of this type of organic material, which currently abounds as waste in the Peruvian highlands. In the specific case of this research, the effect on the stability over time of the biosynthesized silver nanoparticles was evaluated by varying the pH, with values of 4.82, 8.05 and 10.15. It was observed that as the pH increases the production of nanoparticles is higher, having a saturation threshold close to pH 8. It was also found that for alkaline pH close to 10 a more complete reaction of the reducing agent occurs, but with a high dispersion.