A selective liquid medium for direct isolation of Leishmania spp. from mucocutaneous lesions is described. Minimal inhibitory concentrations and synergy studies were performed and used to select 5-fluorocytosine as an antimycotic agent which inhibits contaminating fungi without an adverse effect on the parasite, thus permitting its isolation from contaminated lesions. Penicillin and Streptomycin were included as antibacterial agents. Isolations have been obtained from biopsy material in an average of 5.6 days from 11 of 15 patients with mucous lesions.