The Gulf of Mexico sturgeon Acipenser oxyrinchus desotoi is an anadromous subspecies of the Atlantic sturgeon Acipenser oxyrinchus oxyrinchus. This federally threatened fish once inhabited Gulf coastal rivers from the Mississippi River to Tampa Bay, but is now rarely found south of the Suwannee River in Florida. The Conecuh/Escambia River system of Alabama and Florida contains more potential Gulf sturgeon spawning habitat than any other Florida Panhandle river, yet limited information is known about the sturgeons of this river. Radio transmitters were placed in 14 Gulf sturgeons collected from Escambia Bay during March through May of 2005. These radio-telemetered Gulf sturgeons were manually tracked via boat and radio receivers during April through November 2005 to collect summer holding area and movement data. Two, possibly three, summer holding areas were identified during this study, two in the Escambia River and one in the Delaney River.© 2012 by Nova Science Publishers, Inc. All rights reserved.