Title
Chronic hepatitis B infection is associated with increased molecular degree of inflammatory perturbation in peripheral blood
Date Issued
2020
Access level
open access
Resource Type
journal article
Author(s)
Vinhaes C.L.
Cruz L.A.B.
Menezes R.C.
Carmo T.A.
Queiroz A.T.L.
Barral-Netto M.
Andrade B.B.
Instituto Gonçalo Moniz
Publisher(s)
MDPI AG
Abstract
Hepatitis B virus (HBV) infection remains a major public health concern. The interaction between HBV and the host inflammatory response is an important contributing factor driving liver damage and diseases outcomes. Here, we performed a retrospective analysis employing an adapted molecular degree of perturbation (MDP) score system to assess the overall inflammatory imbalance related to persistent HBV infection. Plasma levels of several cytokines, chemokines, and other inflammatory markers were measured in Brazilian individuals diagnosed with either chronic HBV or previous HBV infection, as well as in uninfected controls between 2006 and 2007. Multidimensional analyses were used to depict and compare the overall expression profile of inflammatory markers between distinct clinical groups. Chronic HBV patients exhibited a marked inflammatory imbalance, characterized by heightened MDP scores and a distinct profile of correlation networks inputting plasma concentrations of the biomarkers, compared with either individuals with previous HBV or controls. Furthermore, in participants with chronic HBV infection, the viral loads in peripheral blood were directly proportional to overall molecular perturbation as well as to specific perturbations of interleukin (IL)-4 and interferon (IFN)-γ concentrations. These findings highlight additional nuances about systemic inflammation related to persistent HBV infection.
Volume
12
Issue
8
Language
English
OCDE Knowledge area
Enfermedades infecciosas
Subjects
Scopus EID
2-s2.0-85089407730
PubMed ID
Source
Viruses
ISSN of the container
19994915
Sponsor(s)
Funding: This work was funded by Financiadora de Estudos e Projetos (FINEP) (grant ward number: 010409605)/Fundo Nacional de Desenvolvimento Científico e Tecnológico (FNDCT-CT-Amazônia) and by the Intramural Research Program of the Fundação Oswaldo Cruz, Brazil. This study was also financed in part by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (Finance Code 001). C.L.V., L.A.B.C., and R.C.M. received research fellowships from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). M.B.A. and T.A.C. received research fellowships from Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). B.B.A. and M.B.-N. are senior investigators from CNPq. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
This work was funded by Financiadora de Estudos e Projetos (FINEP) (grant ward number: 010409605)/Fundo Nacional de Desenvolvimento Científico e Tecnológico (FNDCT-CT-Amazônia) and by the Intramural Research Program of the Fundação Oswaldo Cruz, Brazil. This study was also financed in part by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (Finance Code 001). C.L.V., L.A.B.C., and R.C.M. received research fellowships from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). M.B.A. and T.A.C. received research fellowships from Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). B.B.A. and M.B.-N. are senior investigators from CNPq. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors thank the study participants. In addition, the authors would like to thank Luiz Marcelo Camargo (University of São Paulo, Brazil) for technical and logistic support; Jorge Clarêncio and Daniela Andrade (FIOCRUZ, Brazil) for critical help with the immunoassays, and Jorge Tolentino, Natali Alexandrino, Elze Leite, and Andrezza Kariny for logistic and administrative support.
Sources of information:
Directorio de Producción Científica
Scopus