Title
Characterization of Programmable Transcription Activators in the Model Monocot Setaria viridis Via Protoplast Transfection
Date Issued
01 January 2022
Access level
metadata only access
Resource Type
book part
Author(s)
Sychla A.
CASAS MOLLANO, JUAN ARMANDO
Zinselmeier M.H.
Smanski M.
University of Minnesota
Publisher(s)
Humana Press Inc.
Abstract
Recent advances in DNA synthesis and assembly allow for genetic constructs to be designed and constructed in high throughput. Characterizing large numbers of variant genetic designs is not feasible with low-throughput and time-consuming plant transformation workflows. Protoplast transformation offers a rapid, high-throughput compatible alternative for testing genetic constructs in plant-relevant molecular environments. Here, we describe a protocol for protoplast transformation using a recent experiment in genetic optimization of dCas9-based programmable transcription activators as an example.
Start page
223
End page
244
Volume
2464
Language
English
OCDE Knowledge area
Biología celular, Microbiología Ciencias de las plantas, Botánica
Subjects
DOI
10.1007/978-1-0716-2164-6_16
Scopus EID
2-s2.0-85125975172
PubMed ID
35258836
Resource of which it is part
Methods in Molecular Biology
ISSN of the container
10643745
DOI of the container
10.1007/978-1-0716-2164-6_16
Sponsor(s)
AS and MZ were funded by NIGMS Biotechnology Training grant NIHT32GM008347. MJS and JACM are part of a team supported by the Advanced Plant Technologies program, DARPA Award HR001118C0146. MJS and MZ are supported by USDA NIFA award 2018-33522-28747.
Sources of information: Directorio de Producción Científica Scopus