Bacillus sphaericus 2362 liquid formulation, activity against Anopheles pseudopunctipennis and Culex quinquefasciatus larvae was tested in laboratory bioassays. Breeding place water and distilled water were used with concentrations of 1 x 10", 1 ,5 x 1 O" , 2,5 x 10", 3,9 x 1 O" spores/mL. Target population was kept in laboratory conditions by using breeding native water and flora. One mL doses were added into test cups containing 25 II and III stage larvae and 150 mL of breeding water or distilled respectively; cups tests with the same amount of water and larvae were used as controls for each concentration , water type and larval specie tested. Testing was made three times for each concentration in laboratory conditions. Twelve replicas were made and divided in six for each species. using a total of 4800 larvae for species. Reading of larvae mortality were at 12, 24, 48, and 72 hours after addition of Bacillus sphaericus. It was observed the high susceptibility of Culex quinquefasciatus to Bacillus sphaericus 2362, with an overall mortality > 90% as compared with treated and controls (pvalue = 0,031 and 0,012 for each type of water respectively) at 48 hours and with a concentration of 1,5 x 10" sporesimL. It was widely demonstrated that Anopheles pseudopunctipennís is not susceptible to Bacillus sphaericus 2362 in laboratory bioassays and no significant differences were found in treatments with different types of water (p-value > 0,05).