Title
AmpliBASE MT™: A Mycobacterium tuberculosis diversity knowledgebase
Date Issued
12 April 2004
Access level
metadata only access
Resource Type
journal article
Author(s)
Majeed A.A.
Ahmed N.
Rao K.R.
Ghousunnissa S.
Kauser F.
Bose B.
Nagarajaram H.A.
Katoch V.M.
Cousins D.V.
Sechi L.A.
Hasnain S.E.
J.H. Bloomberg Sch. Publ. Hlth./Hyg
Publisher(s)
Oxford University Press
Abstract
Summary: AmpliBASE MT™ is an online databank of high-resolution DNA fingerprints representing fluorescent amplified fragment length polymorphism (FAFLP) profiles or amplitypes developed for the Mycobacterium tuberculosis complex strains from 48 different countries. AmpliBASE MT™ is based on a relational database management system that is hyperlinked to visualize genotyping results in the form of DNA fingerprint images for individual strains. A flexible search system based on systematic comparisons of fragment sizes in base pairs allows inter-laboratory comparison of FAFLP profiles. Besides this, the database also displays previously published data on IS6110 profiles, spoligotypes, MIRU-VNTRs and large sequence polymorphisms along with the FAFLP records that will give the overall comparisons. Being the first of its kind, AmpliBASE MT™ is expected to be a very helpful tool in strengthening the concept of'geographic genomics'and will be very helpful to molecular epidemiologists and those interested in diagnostic development for tuberculosis. © Oxford University Press 2004; all rights reserved.
Start page
989
End page
992
Volume
20
Issue
6
Language
English
OCDE Knowledge area
Biología celular, Microbiología
Bioquímica, Biología molecular
Epidemiología
Scopus EID
2-s2.0-2342425216
PubMed ID
Source
Bioinformatics
ISSN of the container
13674803
DOI of the container
10.1093/bioinformatics/bth051
Source funding
International Society for Infectious Diseases
World Health Organization
Department of Biotechnology, Ministry of Science and Technology, India
TDR
Sponsor(s)
Financial support from WHO/TDR, International Society for Infectious Diseases (ISID), and core grants to CDFD from the Department of Biotechnology, Government of India is acknowledged. We thank D. van Soolingen and Kristin Kremer for providing a collection of DNA samples from internationally accepted reference strains. Support received from CDFD Bioinformatics team (Ranjit Kumar Gundu, Phani Prasad and Prashanthi) in implementation and hosting the database is acknowledged with thanks. We thank Yogesh Prasad (Applied Biosystems/Labindia) for helpful suggestions and discussions.
Sources of information:
Directorio de Producción Científica
Scopus