The Peruvian industrial fleet deploys digital acoustic technology, mostly commercial echosounders, which must be calibrated when used for quantitative purposes. However no calibration routine is included in the standard version of the operating software of these echosounders, so that a Protocol has been drafted for those purposes. Then, Comparisons with results obtained with scientific echosounders have been made, negligible differences found, so that the use of fishing vessels for monitoring fishing resources constitute a contribution to scientific research carried out by the Peruvian Marine Research Institute (IMARPE) and other entities of the public and private sector. The designed Protocol perform calibration, noise measurement and deletion of interferences in digital Simrad ES60/70 120 kHz echograms. Tests were made aboard eight fishing vessels, also with participation of RV Olaya of IMARPE. Measurements were carried out Callao and Chimbote harbours. The methodology is based on the standard procedures contained in international publications, and is divided into two stages: the first one consists of the recording of acoustic data collected by digital echo sounders during calibration, and the second stage is the processing of acoustic data with algorithms developed in Echoview (Echoview Pty, Tasmania). Calibration.exe software (SIMRAD, Norway) was also used for comparison purposes. The aim of this project is to support the quantitative use of the digital echograms generated by the industrial fleet to detect fish, zooplankton and the upper limit of the minimum oxygen zone (MOZ), also internal structures of the ocean such as internal waves, thermocline and convergent or divergent dynamic vorticity processes.