Title
Fractionation of ethanolic and hydroalcoholic extracts of green propolis using supercritical carbon dioxide as an anti-solvent to obtain artepillin rich-extract
Date Issued
01 August 2018
Access level
metadata only access
Resource Type
journal article
Author(s)
Universidade de Campinas
Publisher(s)
Elsevier B.V.
Abstract
Green propolis from southeastern Brazil is known for its potent biological activity and the presence of Artepillin-C. In order to obtain extracts concentrated in phenolic and artepillin-C compounds, supercritical carbon dioxide (scCO2) was used as an anti-solvent to selectively fractionate ethanolic and hydroalcoholic extracts of green propolis by precipitation in four separators in series. The fractions were evaluated for the global yield of each fraction and for the yields and concentrations of total phenolics (TP), total flavonoids (TF), 3,5-diprenyl-4-hydroxycinnamic acid (Artepillin C) (DHCA), 4-hydroxycinnamic acid (p-coumaric acid) (HCA) and 4-methoxy-3,5,7-trihydroxyflavone (kaempferide) (MTHF) in the fractionated extracts, also evaluated was he antioxidant activity by DPPH. The scCO2 was effective as an anti-solvent to obtain extract fractions rich in Artepillin C and with high antioxidant activity.
Start page
167
End page
173
Volume
138
Language
English
OCDE Knowledge area
Bioproductos (productos que se manufacturan usando biotecnología), biomateriales, bioplásticos, biocombustibles, materiales nuevos bioderivados, químicos finos bioredivados
Salud pública, Salud ambiental
Subjects
Scopus EID
2-s2.0-85046102174
Source
Journal of Supercritical Fluids
ISSN of the container
08968446
Sponsor(s)
The authors thank CNPq for the scholarship awarded to Yaneth Machaca Monroy, and São Paulo Research Foundation FAPESP (Process number 2012/51317-1) for the financial support.
Sources of information:
Directorio de Producción Científica
Scopus