Native/local breeds of chicken are playing major role for the rural poor and marginalized section of the people in developing and underdeveloped countries. The Creole laying hen, comprises a great variety of biotypes, is reared in an extensive system by very small farmers in the high lands and Amazonian region of Peru, is characterized by low production, high resistance to diseases but is not well studied. The objectives of this research were to evaluate the blood biochemical profiles and chemical composition of eggs of Creole and Hy-Line Brown laying hens reared at an extensive and intensive system respectively. Twenty Creole and twenty Hy-Line Brown hens in the laying stage were used; the Creole hens were fed with maize and the Hy-Line Brown hens were fed with a balanced diet according to their requirements. Creole hens shown lower levels of hemoglobin with relation to the Hy-Line Brown and hematocrit levels were higher (p<0.05) for them. Albumin, triglycerides, total cholesterol, high density lipoproteins and low density lipoproteins, were higher (p<0.05) in the Creole hens; meanwhile, the total serum protein was greater (p<0.05) for the Hy-Line Brown hens. Dry matter, ethereal extract and total energy of the eggs without shells and egg yolk were higher (p<0.05) for Creole hens. It is concluded that levels of biochemical profiles and chemical components of the eggs are higher in the Creole than in the Hy-Line Brown lying hens.