Title
Development of Betalain Producing Callus Lines from Colored Quinoa Varieties (Chenopodium quinoa Willd)
Date Issued
17 January 2018
Access level
open access
Resource Type
journal article
Author(s)
Henarejos-Escudero P.
Guadarrama-Flores B.
Guerrero-Rubio M.
García-Carmona F.
Gandía-Herrero F.
Publisher(s)
American Chemical Society
Abstract
Betalains are water-soluble plant pigments of hydrophilic nature with promising bioactive potential. Among the scarce edible sources of betalains is the grain crop quinoa (Chenopodium quinoa Willd), with violet, red, and yellow grains being colored by these pigments. In this work, callus cultures have been developed from differently colored plant varieties. Stable callus lines exhibited color and pigment production when maintained on Murashige and Skoog medium supplemented with the plant growth regulators 6-benzylaminopurine (8.88 μM) and 2,4-dichlorophenoxyacetic acid (6.79 μM) with a reduction of the nitrogen source to 5.91 mM. Pigment analysis by HPLC-DAD and ESI-MS/MS fully describes the content of individual pigments in the cell lines and allows the first report on the pigments present in quinoa seedlings. Phyllocactin and vulgaxanthin I are described as novel pigments in the species and show the potential of C. quinoa culture lines in the production of compounds of nutritional value.
Start page
467
End page
474
Volume
66
Issue
2
Language
English
OCDE Knowledge area
Ciencias de las plantas, Botánica
Subjects
Scopus EID
2-s2.0-85040719035
PubMed ID
Source
Journal of Agricultural and Food Chemistry
ISSN of the container
00218561
Sponsor(s)
This work was supported by “Ministerio de Economıáy Competitividad” (MEC, FSE, Spain) (Project AGL2014-57431) and by “Programa de Ayudas a Grupos de Excelencia de la Regioń de Murcia, Fundacioń Seńeca, Agencia de Ciencia y Tecnologıá de la Regioń de Murcia” (Project 19893/GERM/ 15). B.G.-F. holds a postdoctoral fellowship from “Consejo Nacional de Ciencia y Tecnologıá” (CONACYT, Mexico), M.A.G.-R. holds a contract financed by MEC-FSE (Spain), and P.H.-E. holds a fellowship “Ayudas de Iniciación a la Investigacioń” from the University of Murcia (Spain). Notes The authors declare no competing financial interest.
Sources of information:
Directorio de Producción Científica
Scopus