The available information on the biological diversity of bats in southern Peru is small and fragmented, for that reason developed an inventory of bat species in three provinces of Tacna, Peru. There was a total of 8 species in 15 localities sampled, located in 5 districts in the Tacna Region, distributed as follows Mormopterus kalinowskii and Tadarida brasiliensis exclusively in Ite; Amorphochilus schnablii in Locumba; Desmodus rotundus in Sama; Histiotus macrotus in Ite and Locumba; Platalina genovensium in Sama and Locumba; Histiotus montanus in Sama, Ite and Locumba; Myotis atacamensis in Sama, Pocollay, Ite, Locumba and Chucatamani. The greatest diversity of bats is in Locumba River Basin, with five of the eight species reported.