Objective: To determinate the prevalence of COVID-19 in the nursing personnel, working conditions, and their associated factors in three hospitals in Lima-Peru. Method: A quantitative, descriptive cross-sectional study of the web survey was carried out between November 2020 and February 2021 with 495 nursing personnel members from the three public hospitals in Lima. Results: 63% of the participants were from the Guillermo Almenara Irigoyen National Hospital, 20% from the San Isidro Labrador Hospital, and 17% from the II Vitarte Hospital. The prevalence of COVID-19 in the nursing personnel was 47.3%. When analyzing the demographic characteristics of the participants, a significant association of the education variable with the disease was evidenced (OR=1.50, p=0.04), showing that those with higher education are 1.5 times more likely to develop COVID-19 compared to those with postgraduate studies; the variables related to working conditions and risk factors did not show a significant association (p>0.05) with the disease. Conclusions: Except for education, there is insufficient evidence to affirm a significant association of COVID-19 with demographic characteristics, work-related variables, and those considered a risk. This research makes significant contributions to nursing practice, research, and teaching.