The hematological and blood biochemical parameters are useful tools for equine medical practice but their usefulness for the Peruvian Paso Horse is limited by the absence of reference values. The present study was conducted to determine hemato-biochemical values in apparently healthy horses. Forty nine animals were evaluated and they were classified according to sex [males (n=17), females (n=30)] and age [young (n=23), adults (n=26)]. The hematological (red blood cell count, hematocrit, hemoglobin concentration, mean corpuscular volume, mean corpuscular hemoglobin concentration, white blood cell count, band neutrophils, segmented neutrophils, eosinophils, monocytes, lymphocytes, neutrophil/lymphocyte ratio and platelet count) and the biochemical (total bilirubin, direct bilirubin, indirect bilirubin, ALT, AST, ALP, GGT and CK) profiles were estimated by means of automated analyzers. The effect of sex and age was evaluated. Sex influenced the number of monocytes and CK activity, and age influenced hemoglobin concentration, mean corpuscular volume, mean corpuscular hemoglobin concentration, lymphocytes, neutrophil/lymphocyte ratio, ALT, AST, total bilirubin and indirect bilirubin (p<0.05).