Title
Soft rot in organic banana pseudostem (Musa sp): Symptomatology, cultural and biochemical characterization, pathogenicity, and management alternatives
Date Issued
01 December 2021
Access level
open access
Resource Type
journal article
Author(s)
Ruiz W.R.
Tirado-Lara J.
Saucedo-Bazalar M.
Tuesta-Albán C.
Apaza-Apaza S.
Teodor K.K.
Publisher(s)
Universidad Nacional de Trujillo
Abstract
The banana (Musa sp.) is a very important crop in the socioeconomic development of Peru. The soft rot disease has been causing economic losses. The objective was to isolate and identify the causal agent of soft rot in banana and to implement management alternatives. Bacteria were isolated from pseudostem with soft rot symptoms from 11 producing areas in the Chira Valley. The pathogenicity tests were carried on pseudostem segments and banana shoots. The eight isolates were culturally, morphologically and biochemically characterized: All isolates were Gram negative, rod-shaped, with peritrichous flagella and colonies formed deep cavities on crystal violet pectate (CVP) medium. Biochemical tests showed a positive catalase reaction, gelatin hydrolysis, glucose and lactose fermentation, and tolerance to NaCl 5%. However, on TSI (Triple Iron Sugar) medium and erythromycin sensitivity only isolates A2M, A3Ma, A5Mall, A7VF, A9Sa and A10 reacted positively and isolates A6Q and A11Sal negatively. Growth at 28, 30, 36, 36, 37 and 45°C was positive for all eight isolates, with the exception A6Q and A11Sal which did not develop at 45°C. Cultural characteristics, biochemical and pathogenicity tests allowed to identify six isolates as Pectobacterium chrysanthemi and two as P. carotovorum subsp. carotovorum. Disease management is based on the use of certified suckers, adequate fertilization, organic matter applications, biocontrol agents and weevil insect control.
Start page
571
End page
578
Volume
12
Issue
4
Language
Spanish
OCDE Knowledge area
Métodos de investigación bioquímica
Ciencias de las plantas, Botánica
Protección y nutrición de las plantas
Subjects
Scopus EID
2-s2.0-85122581044
Source
Scientia Agropecuaria
ISSN of the container
20779917
Sources of information:
Directorio de Producción Científica
Scopus