The construction of amperometric enzymeless biosensors for phenolic compounds determination, using carbon paste electrode modified with copper phtalocyanine (CuPc) and histidine (His), based on the chemistry of the dopamine β-monooxygenase (DβM) enzyme that catalyzes the hydroxylation of the dopamine and its analogs is shown. The modified carbon paste was evaluated on electrodes constructed in two ways: putting the paste into a cavity of a rotating disk electrode and a platinum slide electrode fixed into a glass tube. The sensor in hydrodynamic conditions presented a linear response range between 30 and 250 μmoll-1, with a sensitivity of 4.6 ± 0.1nAl μmol-1 cm-2 for catechol, response time of 3 s and lifetime of about 50 days when stored at room temperature. The sensor in static conditions showed a linear response range from 40 to 250 μmoll-1, with a sensitivity of 0.30 ± 0.01nAl μmol-1 cm-2 for catechol. The sensors presented the following relative response order for dopamine and some analog species: catechol > dopamine > guaiacol > serotonin > phenol. © 2002 Elsevier Science B.V. All rights reserved.