Title
Effect of two antioxidants (tempo and tempol) on Ram Semen cryopreservation using the tris extender
Other title
Efecto de dos antioxidantes (tempo y tempol) en la criopreservación de semen ovino empleando un dilutor en base a tris
Date Issued
01 January 2007
Access level
metadata only access
Resource Type
journal article
Author(s)
Wilfredo Huanca L.
César Alzamora P.
Publisher(s)
Universidad Nacional Mayor de San Marcos
Abstract
Thirty-two semen samples from four rams were frozed using two antioxidants, Tempo (2,2,6,6 tetramethyl-1-piperidinyloxyl) and Tempol (4-hidroxi 2,2,6,6 tetramethyl-1- piperidinyloxyl), each one in concentrations of 0.5, 1.0, and 2.5 mM, to evaluate the effects on post-thawing motility, viability, acrosomal integrity, and earlier sperm capacitation. Eight replications for each antioxidant concentration and control group, were done using four semen samples per replication. Semen samples were diluted on a Tris extender. Results showed that Tempo 0.5 mM improved frozen semen quality in comparison with control group (p<0.05), by increasing progresive motility (79 vs. 67%), viability and acrosomal integrity (70 vs. 58%), and decreasing earlier sperm capacitation (9 vs. 15%). On the other hand, Tempol decreased frozen semen quality. In conclusion, the use of Tempo 0.5 mM on a Tris extender, at the end of the cooling process, could be an alternative for improving the quality of frozed ram semen.
Start page
99
End page
106
Volume
18
Issue
2
Language
Spanish
OCDE Knowledge area
Ciencia veterinaria
Subjects
Scopus EID
2-s2.0-84872754526
Source
Revista de Investigaciones Veterinarias del Peru
ISSN of the container
16823419
Sources of information:
Directorio de Producción Científica
Scopus