Title
Determination of the neutralizing potency of horse antibothropic and anticrotalic antivenoms in blood samples collected on filter paper
Date Issued
18 August 2001
Access level
metadata only access
Resource Type
journal article
Author(s)
Fundaçao Ezequiel Dias
Abstract
The correlation coefficients between in vivo neutralization of lethal toxicity (ED50) and levels of antibodies measured by enzyme-linked immunosorbent assay (ELISA) in blood samples collected on filter paper were investigated to test the potency of horse antibothropic and anticrotalic antivenoms. Sixteen horses were hyperimmunized with Bothrops venom (50% from B. jararaca and 12.5% each from B. alternatus, B. jararacussu, B. neuwiedii and B. moojeni) and 12 horses with Crotalus durissus terrificus venom. Crude venom of C. d. terrificus and the lethal fraction of B. jararaca venom were used as antigens to set up an indirect ELISA. The correlation coefficient between ED50 and ELISA antibodies titers against C. d. terrificus and the lethal fraction of B. jararaca venom was r = 0.8 (P < 0.001) and r = 0.78 (P < 0.001), respectively. © 2001 Elsevier Science Ltd. All rights reserved.
Start page
1607
End page
1609
Volume
39
Issue
10
Language
English
OCDE Knowledge area
Toxicología
Subjects
Scopus EID
2-s2.0-0034904787
PubMed ID
Source
Toxicon
ISSN of the container
00410101
Sponsor(s)
Funding text
This research was supported by grants from Conselho Nacional de Desenvolvimento Cientı́fico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG). C. Chávez-Olórtegui is the recipient of a CNPq fellowship.
Sources of information:
Directorio de Producción Científica
Scopus