Title
Cloning and characterization of dnaE, encoding the catalytic subunit of replicative DNA polymerase III, from Vibrio cholerae strain C6706
Date Issued
10 October 1996
Access level
metadata only access
Resource Type
journal article
Author(s)
Franco A.A.
Yeh P.E.
Johnson J.A.
Barry E.M.
GUERRA ALLISON, ANTONIO HUMBERTO
Maurer R.
Morris J.G.
Universidad Peruana Cayetano Heredia
Publisher(s)
Elsevier B.V.
Abstract
We report that Vibrio cholerae (Vc) contains a gene homologous to Escherichia coli dnaE, the structural gene for the a (catalytic) subunit of replicative DNA polymerase III (PolIII). Despite 24% amino acid (aa) differences in the encoded proteins, the Vc gene strongly complements an E. coli dnaE temperature sensitive (ts) mutant, indicating that all functional features essential for replication are conserved.
Start page
281
End page
283
Volume
175
Issue
February 1
Language
English
OCDE Knowledge area
Biología celular, Microbiología Bioquímica, Biología molecular
Subjects
DOI
10.1016/0378-1119(96)00155-2
Scopus EID
2-s2.0-0030579152
PubMed ID
8917113
Source
Gene
ISSN of the container
03781119
Sponsor(s)
National Institute of General Medical Sciences R01GM047111 NIGMS
Sources of information: Directorio de Producción Científica Scopus