Bread crumb microstructure was quantified by using cryo-scanning electron microscopy (50× - 150×) and results were compared to the values normally obtained from macroscopical images using a digital camera imaging system. Whereas crumb void fraction was underestimated in the results from the camera images (~0.45), the intercellular distance was overestimated (~0.86 mm). Actual values of void fraction and intercellular distance were on average 0.75 and 66 μm, respectively. Tiny cells of 10-80 μm were mostly located within the cell walls.