The objective of the research was to evaluate the inhibition of lichen and moss growth present in the lithic elements used in the construction of facades of patrimonial Cusco churches by applying air nanobubbles. For this study, a sample of 20 lithic elements from the original quarries was taken to avoid experimenting with stone elements from said churches. The length of each sample was in the range of 7 to 10 cm with the presence of biotic agents; the application of air nanobubbles was carried out for periods of 10 minutes. Once the experiment was over, the samples were exposed for 04 weeks to climatic factors such as rain, temperature, and natural atmospheric pressure. In the qualitative results, it is observed that the samples with exposure of more than 10 min. they achieved detachments and drying of the biotic agent. From the analyzed photographs, it can be concluded that the air nanobubbles continue to affect over long periods in the analyzed samples to the point of eliminating the pathology, while in the control sample their propagation and growth are observed.