Title
Detection, quantification and morphological characterization of vibrio cholerae indicator bacteriophages
Other title
[Detección, cuantif1cación ycaracterización morfológica de bacteriófagosindicadores de vibrio cholera]
Date Issued
01 January 1998
Access level
open access
Resource Type
journal article
Publisher(s)
Universidad Nacional Mayor de San Marcos, Fac. Of Biological Sci.
Abstract
The specificity between bacteriophages and bacteria is a feature that has been successfully used for the detection of several microbial species. For this reason the detection of vibriophages is a valuable research tool, and it might be a fast and convenient method to Vibrio cholerae diagnostics. The goal of this study was to detect vibriophages in shallow seawater samples and to determine the morphological characteristics of these vibriophages. It was determined the growth kinetics of a strain of Vibrio cholerae serotype Inaba. Seawater samples taken from five shallow points from an adjacent sector to La Chira beach, and Rimac and Chillon river mouths were analysed qualitatively and quantitatively using different inoculum volumes and several incubation periods. The bacteriophages were concentrated and stained for the morphological study by transmission electron microscopy. The results we obtained indicate that the vibriophages could be an important tool as a Vibrio cholerae indicator.
Start page
90
End page
97
Volume
5
Issue
2
Language
Spanish
OCDE Knowledge area
Patología Biología celular, Microbiología
Scopus EID
2-s2.0-84872122741
Source
Revista Peruana de Biologia
ISSN of the container
15610837
Sources of information: Directorio de Producción Científica Scopus