Streptomyces sp. has the potential to inhibit the incidence of fungal phytopathogens in crops due to their biochemical activity and the production of secondary metabolites. This work was aimed to identify and evaluate in vitro the antifungal potential of five strains of Streptomyces sp., isolated from desert soil, against Colletotrichum sp. The isolated strains were morphologically characterized by macroscopic and microscopic analysis, and their inhibition percentage against Colletotrichum sp. were determined by the dual challenge and well diffusion tests, applying a completely randomized design with three replicates. The inhibitory capacities of the five strains versus the phytopathogenic fungus varied considerably. Strains Q-84 and Q-60 showed the highest percentages of inhibition in the dual challenge test, with 92 and 88 %, respectively. In the well diffusion test, the crude bioactive extract of strain Q-84 showed the highest inhibition (56 %) statistically similar to that of commercial chemical fungicides AmistarTop® and Opera® (84 and 100 %, respectively). All the strains displayed lipase activity and three of them (Q-84 included) also showed protease activity. The genetic characterization by 16S rDNA sequencing evidenced the homology of the strain Q-84 DNA to that of S. maritimus, further coincident with the lipase activity profile. Overall, the Q-84 showed potential to be used as biocontrol against Colletotrichum sp.