Plants were regenerated from axillary shoot tips of 12 Andean potato clones, chosen to cover the full range of ploidies from 2X to 5X, and one European tuberosum clone. Using Murashige and Skoog (MS) medium supplemented with 0.1 mg/l gibberellic acid (GA) and naphthaleneacetic acid (NAA) (singly or in combination), single-shoot cultures were initiated from 20-30% of the tuberosum explants but from only 5-10% of explants from the Andean clones. With the latter clones, multiple-shoot cultures were produced from 50-100% of the explants when they were allowed to produce a small basal callus on MS medium supplemented with 3.0 mg/l benzylaminopurine (BAP), before being transferred to MS medium supplemented with 0.1 mg/l GA. Once roots were formed, plantlets could be readily established in soil. © 1977.