Title
Activity-guided isolation of the chemical constituents of Muntingia calabura using a quinone reductase induction assay
Date Issued
01 January 2003
Access level
metadata only access
Resource Type
journal article
Author(s)
Su B.
Parka E.
Graham J.
Fong H.
Pezzuto J.
Kinghorn A.
Publisher(s)
Elsevier Ltd
Abstract
Activity-guided fractionation of an EtOAc-soluble extract of the leaves of Muntingia calabura collected in Peru, using an in vitro quinone reductase induction assay with cultured Hepa 1c1c7 (mouse hepatoma) cells, resulted in the isolation of a flavanone with an unsubstituted B-ring, (2R,3R)-7-methoxy-3,5,8-trihydroxyflavanone (5), as well as 24 known compounds, which were mainly flavanones and flavones. The structure including absolute stereochemistry of compound 5 was determined by spectroscopic (HRMS, ID and 2D NMR, and CD spectra) methods. Of the isolates obtained, in addition to 5, (2S)-5-hydroxy-7-methoxyflavanone, 2′,4′-dihydroxychalcone, 4,2′,4′-trihydroxychalcone, 7-hydroxyisoflavone and 7,3′,4′-trimethoxyisoflavone were found to induce quinone reductase activity. © 2003 Elsevier Science Ltd. All rights reserved.
Start page
335
End page
341
Volume
63
Issue
3
Language
English
OCDE Knowledge area
Ciencias de las plantas, Botánica
Farmacología, Farmacia
Subjects
Scopus EID
2-s2.0-0038445374
PubMed ID
Source
Phytochemistry
ISSN of the container
0031-9422
Sponsor(s)
This work was supported by Program Project P01 CA48112 funded by the National Cancer Institute, NIH, Bethesda, MD. We are grateful to Drs. J. A. (Art) Anderson, Research Resources Center, University of Illinois at Chicago, and K. Fagerquist, Mass Spectrometry Facility, Department of Chemistry, University of Minnesota, Minneapolis, MN, for mass spectral data.
Sources of information:
Directorio de Producción Científica
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