The sperm hyperactivation is a step of the spermatozoa capacitation and consists on a change in the pattern of movements, the variation of the angle of the head, and the longitude and width of the movement of the tail. The objective of the present study was to determine the difference in movement patterns of spermatozoa and the frequency of hyperactive spermatozoa in the oviduct according to the time after mating. The recovery of the spermatozoa in five alpacas was done after 16, 20, 24, 28 and 32 hours post mating. Sperms were evaluated according to the pattern of movement. The frequency of hyperactive spermatozoa increased until 28 hours post copula (71.4%).