Title
Chagas disease in Southern Coastal Ecuador: Coinfections with arboviruses and a comparison of serological assays for chagas disease diagnosis
Date Issued
01 January 2018
Access level
open access
Resource Type
journal article
Author(s)
Mita-Mendoza N.K.
McMahon E.
Kenneson A.
Barbachano-Guerrero A.
Beltran-Ayala E.
Cueva C.
King C.A.
Lupone C.D.
Endy T.P.
Stewart-Ibarra A.M.
Johns Hopkins Bloomberg School of Public Health
Johns Hopkins Bloomberg School of Public Health
Publisher(s)
American Society of Tropical Medicine and Hygiene
Abstract
Occurrence of Chagas disease and arbovirus coinfections is unknown, despite the vast co-endemic areas throughout the Americas. This study examined the proportion of individuals positive for Trypanosoma cruzi and coinfections with dengue, chikungunya, and Zika viruses in Machala, Ecuador (January 2014–December 2015). Chagas seropositivity was evaluated with five commercially available assays. Dengue infections were identified by nonstructural protein 1 rapid test and enzyme linked immunosorbent assay (ELISA), immunoglobulin M ELISA, and reverse transcription PCR (RT-PCR); chikungunya and Zika infections were identified by RT-PCR. Of 658 individuals, six were positive for T. cruzi (0.91%), including one T. cruzi/dengue coinfection and one T. cruzi/chikungunya/dengue coinfection. The clinical manifestations of coinfected individuals corresponded to severe dengue and dengue with warning signs, respectively. We observed discrepant results by using the Hemagen Chagas kit and the rapid test Chagas Detect Plus (false positives: 3.9% and 15.4%), highlighting the need to assess diagnostic assays in geographic regions with distinct taxonomic units of T. cruzi.
Start page
1530
End page
1533
Volume
99
Issue
6
Language
English
OCDE Knowledge area
Enfermedades infecciosas
Scopus EID
2-s2.0-85058164698
PubMed ID
Source
American Journal of Tropical Medicine and Hygiene
ISSN of the container
00029637
Sponsor(s)
U.S. Department of Defense - P0220_13_OT - DOD
Financial support: Y. C. S. reports grants and nonfinancial support from Inbios International, other from Hemagen, during the conduct of the study; and grants and nonfinancial support from Inbios International outside the submitted work. E. M. reports grants from Syracuse University, during the conduct of the study.
Acknowledgments: This study was supported in part by the Department of Defense Global Emerging Infection Surveillance (GEIS) grant (P0220_13_OT) and the Department of Medicine of SUNY Upstate Medical University. A. M. S. was additionally supported by NSF DEB EEID 1518681 and NSF DEB RAPID 1641145; E. M. by a Syracuse University Renee Crown Honors Program Crown Award; and A. B.-G. by a fellowship from the Mexican Council for Science and Technology (CONACYT). Many thanks to Ortho Clinical Diagnostics for the donation of the ORTHO T. cruzi ELISA test system kits.
Sources of information:
Directorio de Producción Científica
Scopus