Title
Influence of DNA-repair gene variants on the micronucleus frequency in thyroid cancer patients
Date Issued
20 January 2013
Access level
metadata only access
Resource Type
journal article
Author(s)
Universitat Autònoma de Barcelona
Abstract
The role of different DNA-repair genes (OGG1, XRCC1, XRCC2 and XRCC3) on both the spontaneous and the induced frequency of micronuclei (MN) has been studied in the lymphocytes of a group of 114 patients with differentiated thyroid cancer (DTC). Induction of MN was achieved by treatment of the lymphocytes with 0.5. Gy of gamma-radiation.The selected genes are involved in base-excision repair (BER) (OGG1, Ser326Cys; XRCC1, Arg280His and Arg399Gln), and in homologous recombination repair (HRR) (XRCC2, Arg188His and XRCC3, IVS5-14G). Genotyping was carried out by use of the iPLEX (Sequenom) technique.Results indicate that only the OGG1-Ser326Cys polymorphism was able to modulate the MN frequency. This effect was only observed in the spontaneous MN frequency (P=0.016), but not in the MN frequency induced after irradiation. In addition, a strong correlation was observed between spontaneous and induced MN frequency, which would suggest an underlying genetic background. © 2012 Elsevier B.V.
Start page
34
End page
39
Volume
750
Issue
February 1
Language
English
OCDE Knowledge area
Bioquímica, Biología molecular
Oncología
Genética, Herencia
Subjects
Scopus EID
2-s2.0-84871716585
PubMed ID
Source
Mutation Research - Genetic Toxicology and Environmental Mutagenesis
ISSN of the container
18793592
DOI of the container
10.1016/j.mrgentox.2012.08.008
Source funding
Pilot International Foundation
Sources of information:
Directorio de Producción Científica
Scopus