Title
Rapid plant DNA and RNA extraction protocol using a bench drill
Date Issued
01 January 2019
Access level
open access
Resource Type
journal article
Author(s)
Ferreira C.F.
Iskra-Caruana M.L.
Chabannes M.
Barbosa A.C.O.
Santos T.A.
Silva A.G.S.
Santos R.M.F.
Amorim E.P.
de Oliveira S.A.S.
Jesus O.N.
Publisher(s)
Fundacao de Pesquisas Cientificas de Ribeirao Preto
Abstract
Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and RNA extraction that uses an inexpensive bench drill and plastic bags and does not require liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethylammonium bromide method. Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. This protocol can reduce costs considerably in laboratories with ongoing routine activities of DNA and RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic acids. This is especially relevant for many laboratories in developing countries where the cost and availability of liquid nitrogen may be a constraint.
Volume
18
Issue
3
Language
English
OCDE Knowledge area
Ciencias de las plantas, Botánica
Tecnología de modificación genética
Subjects
DOI
Scopus EID
2-s2.0-85073268263
Source
Genetics and Molecular Research
Sponsor(s)
The authors would like to thank the support from Embrapa Mandioca e Fruticultura, the Bill and Melinda Gates Foundation, Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB) and Conselho Nacional do Desenvolvimento Científico e Tecnológico (CNPq).
Sources of information:
Directorio de Producción Científica
Scopus