OBJECTIVES: Knowing the current prevalence of hepatitis C virus infection has taken great importance nowdays due to the new advances in the treatment of hepatitis C virus infection in which the new direct-acting antivirals have demonstrated a high rate of sustained viral response. MATERIALS AND METHODS: This is a descriptive study based on the detection of anti VHC in the blood banks of “Ministerio de Salud del Perú†according to the information obtained from “Programa Nacional de Hemoterapia y Bancos de Sangre†(PRONAHEBAS). RESULTS: In our study, the prevalence of anti-hepatitis C antibodies in blood donors nationwide was 0.428% in 2016 and 0.301% in 2017. CONCLUSION: To know updated data about the prevalence of hepatitis C virus infection in our population. It is the first step to be able to treat the disease and drastically reduce the infection rate according to the WHO target for 2030.