The most widely used feed ingredients in the world are fishmeal and soybean, which, despite having high-quality digestible protein and good fat content, are considered environmentally unsustainable and increasingly expensive. This issue also involves the guinea pig, a very important animal protein source for people in Andean regions in South America. Here we investigate the substitution of soybean meal with 50% and 100% black soldier fly larvae meal in the guinea pig diet and its effects on meat quality (fatty acid profile, amino acid profile, water-holding capacity, pH, proximal composition, and color). The results showed no differences in the protein content and amino acid profile of meat nor in the n-6:n-3 and P/S ratios, but did show an increment in the desirable fats (mono-and polyunsaturated fatty acids) in the guinea pigs fed with black soldier fly larvae meal. All the other analyzed parameters showed no differences among the diets tested. These results suggest that total replacement of soybean meal with black soldier fly larvae meal in guinea pig nutrition is feasible since meat quality was maintained or improved.