The promotion of the research culture at the level of the countries and their educational institutions, uses the design and development of programs that allow strengthening the investigative skills, including the cognitive, procedural and attitudinal skills of its staff. This research is part of the program to promote research culture in Peru carried out in universities in the southern area of ​Lima, to strengthen their university teachers. The objective of the was to determine the influence of the program promoting the research culture in the strengthening of teacher competencies. To do this, he followed the hypothetical deductive method, with a quantitative approach, based on applied research, experimental design, quasi-experimental sub-design, and cross section. The population was made up of 520 university teachers from the south of Lima, the sample was 212 teachers. The results are framed in the experimental group, 1.9% shows a good level in terms of the consolidation of investigative skills, 86.8% a very good level, while 11.3% has an excellent level. It is concluded that there is a favorable influence of the program promoting the research culture, managing to strengthen the investigative skills studied in teachers, this manifested in the increase in their academic and scientific productivity.