Title
In vitro embryo production in wood bison (Bison bison athabascae) using in vivo matured cumulus-oocyte complexes
Date Issued
01 February 2017
Access level
metadata only access
Resource Type
journal article
Author(s)
University of Saskatchewan
Publisher(s)
Elsevier Inc.
Abstract
Experiments were conducted in wood bison to determine the effect of additional maturation time on embryo development of in vivo matured oocytes. In experiment 1, cumulus-oocyte complexes (COC) were collected 30 hours after hCG treatment in superstimulated wood bison, and expanded COC were fertilized immediately or after 4 hours of additional in vitro maturation. Embryo development was assessed on Days 3, 7, and 8 (Day 0 = day of fertilization). No difference in cleavage rate was detected (55.3% vs. 60.5%, P = 0.82), but the Day 8 blastocyst rate was higher after an additional 4 hours of in vitro maturation time (44.7 vs. 18.4%, P = 0.03). In experiment 2, COC were collected at either 30 hours or 34 hours after hCG treatment. Expanded COC from the 30 hours group were fertilized after 4 hours of in vitro maturation, whereas those from the 34 hours group were fertilized immediately. A higher cleavage rate (74.3 vs. 57.0%) and blastocyst rate (54.1 vs. 37.2%) were found in the 34 hours group versus the 30 hours group (P < 0.05). In conclusion, an additional short period of in vitro maturation, or an extended period of in vivo maturation are beneficial for in vitro embryo production in wood bison.
Start page
122
End page
130
Volume
89
Language
English
OCDE Knowledge area
Biología reproductiva
Conservación de la Biodiversidad
Scopus EID
2-s2.0-84995750530
PubMed ID
Source
Theriogenology
ISSN of the container
0093691X
Sponsor(s)
This study was supported by grants from the University of Saskatchewan , the Government of the Northwest Territories , Parks Canada , and Provincial and National Bison Associations . The authors gratefully acknowledge Elk Island National Park as the original source of the wood bison used in the study, and Dr. Murray Woodbury (Agriculture and Agri-Food Canada Specialized Livestock Chair, University of Saskatchewan) for sustaining the bison research program. We thank Drs. Rodrigo Carrasco, Elsie Dawn, and Serena Caunce and also to Mary von der Porten for help with data collection, Vetoquinol NA Inc. for providing FSH (Folltropin-V), pLH (Lutropin-V), and hyaluronan (MAP-5), and Merck Animal Health for providing hCG (Choluron) and prostaglandin analogous (Estrumate). We also thank the farm staff at the Native Hoofstock Centre, University of Saskatchewan for maintaining the animals used in this study. MPC participated in designing the study, acquisition, analysis and interpretation of data, and in writing and revising the manuscript. JMP participated in acquisition, analysis, and interpretation of data, and helped to draft the manuscript. MA, RJM, and GM participated in the study design and in revising the manuscript. As principal investigator and senior author, GPA provided the intellectual impetus for this study, and contributed to the experimental design, analysis, and interpretation, as well as writing and revising the manuscript.
Sources of information:
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