Title
Quadratic blind linear unmixing: A graphical user interface for tissue characterization
Date Issued
01 February 2016
Access level
open access
Resource Type
journal article
Author(s)
Texas A andM University
Publisher(s)
Elsevier Ireland Ltd
Abstract
Spectral unmixing is the process of breaking down data from a sample into its basic components and their abundances. Previous work has been focused on blind unmixing of multi-spectral fluorescence lifetime imaging microscopy (m-FLIM) datasets under a linear mixture model and quadratic approximations. This method provides a fast linear decomposition and can work without a limitation in the maximum number of components or end-members. Hence this work presents an interactive software which implements our blind end-member and abundance extraction (BEAE) and quadratic blind linear unmixing (QBLU) algorithms in Matlab. The options and capabilities of our proposed software are described in detail. When the number of components is known, our software can estimate the constitutive end-members and their abundances. When no prior knowledge is available, the software can provide a completely blind solution to estimate the number of components, the end-members and their abundances. The characterization of three case studies validates the performance of the new software: ex-vivo human coronary arteries, human breast cancer cell samples, and in-vivo hamster oral mucosa. The software is freely available in a hosted webpage by one of the developing institutions, and allows the user a quick, easy-to-use and efficient tool for multi/hyper-spectral data decomposition.
Start page
148
End page
160
Volume
124
Language
English
OCDE Knowledge area
Biotecnología médica
Radiología, Medicina nuclear, Imágenes médicas
Subjects
Scopus EID
2-s2.0-84954530034
PubMed ID
Source
Computer Methods and Programs in Biomedicine
ISSN of the container
01692607
Sponsor(s)
The authors acknowledge Dr. Melissa Skala and Alex Walsh, from the Department of Biomedical Engineering at Vanderbilt University, for providing the FLIM dataset of human breast cancer cells employed in Section 5.2 . This research was supported by grants from CONACYT-TAMU ( 2012-034 ) and NIH ( R01CA138653 , R01HL11136 ). The work of O. Gutierrez-Navarro and D.U. Campos-Delgado was supported by CONACYT and COMEXUS Fulbright-Garcia Robles fellowships.
Sources of information:
Directorio de Producción Científica
Scopus