Over the last decade, Microbial Fuel Cells (MFCs) have experienced significant scientific and technological development, to the point of becoming close to commercialization. One key assessment that clearly establishes whether one technology can fully enter the market is the profitability demonstration. For this demonstration, classical evaluation criteria for investment decisions such as the Net Present Value and the Internal Rate of Return can be applied to a given proposal. This paper presents an economic assessment of a microbial fuel cell in a juice processing plant. Three different scenarios, optimistic, pessimistic and most likely scenarios based on the maximum power density of the cell on two basic MFC cases (cathodes with and without Pt, respectively), were studied and compared to the conventional activated sludge process. The results show that under most of the scenarios under consideration, including the pessimistic one, MFC is a more attractive option. Furthermore, a sensitivity analysis was performed with respect to the electrode area, and the annual growth rate of the electricity pricing has revealed that the electrode area parameter is the most influential, reducing the MFC profitability for larger electrode areas, whereas the higher the annual growth rates of the electricity price, the higher the MFC profits. In summary, the results of this study show that the implementation of MFC is a promising alternative to the use of classical aerated activated sludge, and it has potential economic benefits.