The objective of this study was to investigate the enzymatic generation of the Mn3+-malonate complex and its application to the process of oxidizing several organic compounds. The experimental set-up consisted of an enzymatic reactor coupled to an ultrafiltration membrane, providing continuous generation of Mn3+-malonate from a reaction medium containing versatile peroxidase (an enzyme produced by Bjerkandera adusta strain BOS55), H2O2, MnSO4, and malonate. The effluent of the enzymatic reactor was introduced into a batch-stirred reactor to oxidize three different classes of compounds: an azo dye (Orange II), three natural and synthetic estrogens, and a polycyclic aromatic hydrocarbon (anthracene). The enzymatic reactor provided the Mn3+ complex under steady-state conditions, and this oxidative species was able to transform the three classes of xenobiotics considerably (90-99%) with negligible loss of activity. © 2011 American Institute of Chemical Engineers (AIChE).