A new aminosteroid, 3β-amino-22,26-epiminocholest-5-ene named sarachine (1), and two known flavonoids, eriodictyol (2) and 7-O-β-D-glucopyranosyl- eriodictyol (3), were isolated from the leaves of Saracha punctata. The alkaloid was found to inhibit the growth of Leishmania braziliensis promastigotes (100% at 25 μM) and of Trypanosoma cruzi epimastigotes in culture (50% at 25 μM) and showed a strong in vitro antiplasmodial activity with an IC50 of 25 nM.