Title
Role of lipopolysaccharide in the induction of type I interferon-dependent cross-priming and IL-10 production in mice by meningococcal outer membrane vesicles
Date Issued
18 March 2009
Access level
metadata only access
Resource Type
journal article
Author(s)
Center of Molecular Immunology
Abstract
We investigated the contribution of lipopolysaccharide (LPS) to adjuvant properties of native outer membrane vesicles (NOMV), a vaccine candidate for meningococcal B disease. NOMV induce the maturation of and cytokine production by murine bone marrow-derived dendritic cells through both toll-like receptors (TLR) 2 and 4 which are mostly dependent on the signalling adaptor MyD88. NOMV are also able to induce B cell proliferation in splenocytes from LPS-hyporesponsive mice. However, induction of IL-10 and type I interferon-dependent, antigen-specific and IFNγ-secreting CD8+ cytotoxic T lymphocyte responses in vivo by NOMV requires LPS. The importance of LPS in the induction of IL-10 and functional cross-priming has implications for NOMV-based vaccine and adjuvant development. © 2009 Elsevier Ltd. All rights reserved.
Start page
1912
End page
1922
Volume
27
Issue
13
Language
English
OCDE Knowledge area
Inmunología
Subjects
Scopus EID
2-s2.0-61349186919
PubMed ID
Source
Vaccine
ISSN of the container
0264410X
Sponsor(s)
We thank Dr. Svein Rune Andersen and Mr. Geoffrey Guile for preparing Nm cell membranes and NOMV and performing LPS quantification, and the Edward Jenner Institute for Vaccine Research for funding this work. The Jenner Institute was sponsored by the U.K. Department of Health, Biotechnology and Biological Sciences Research Council, GlaxoSmithKline and Medical Research Council. We also thank IAH Compton, Berkshire, U.K. for co-sponsoring a studentship for V. Durand.
Sources of information:
Directorio de Producción Científica
Scopus