Title
Ascaris suum lung larvae antigens recognized by antibodies produced in Oryctolagus cuniculus
Other title
Antígenos de larvas pulmonares de Ascaris suum reconocidos por anticuerpos producidos en Oryctolagus cuniculus
Date Issued
01 December 2005
Access level
metadata only access
Resource Type
journal article
Publisher(s)
Universidad de Chile
Abstract
Excretory/secretory antigens (E/SAg) and somatic antigens (SAg) of Ascaris suum lung larvae that induce the immunoglobulin G antibodies production in Oryctolagus cuniculus experimentally immunized was determined. For this purposes, specimens of Mus musculus BALB/c were inoculated orally with infective eggs of A. suum obtained from pigs naturally parasitized in order to obtain the lung larvae. Part of these larvae was cultured in Minimum Essential Medium (MEM) to obtain E/SAg and another part was sonicated to obtain the SAg too. Both, E/SAg and SAg mixed with complete and incomplete Freund's adjuvant were used for rabbits immunization. Five weeks after the immunization, the rabbits were bled by cardiac puncture obtaining the immunosera by centrifugation, which was purified partially by saline precipitation and dialysis. By using an Western blot technique with purified immunoserum and E/SAg obtained to 20 hours and reduced with dithiothreitol, fourteen antigens bands of 100, 72.4, 56.2, 42.7, 39.8, 34.6, 31.6, 30.2, 19.5, 16.9, 15.5 y 14.9 KD, were detected. The bands of 100, 72.4, 16.9, 15.5 and 14.9 KDa were been the most reactives. Thereby the SAg, also reduced with dithiothreitol, seven bands of 42.7, 39.8, 34.6, 30.2, 28.0 and 25.2 KDa were detected. They were a bit clear. In conclusion, the E/SAg induce the highest production of immunoglobulin G antibodies in rabbits experimentally immunized.
Start page
132
End page
137
Volume
60
Issue
April 3
Language
Spanish
OCDE Knowledge area
Bioquímica, Biología molecular Parasitología
Scopus EID
2-s2.0-55449133092
Source
Parasitologia Latinoamericana
ISSN of the container
07177704
Sources of information: Directorio de Producción Científica Scopus