Title
Molecular detection of taeniid eggs in beetles collected in an area endemic for Taenia solium
Date Issued
01 January 2018
Access level
open access
Resource Type
journal article
Author(s)
Publisher(s)
American Society of Tropical Medicine and Hygiene
Abstract
The aim of this study was to demonstrate the presence of Taenia solium eggs in beetles collected from sources within the natural environment through molecular techniques. Fifty-four pools of beetles were collected in three villages in Piura, Peru. DNA was extracted using the FastDNA spin kit for soil. Molecular identification of Taenia species was then performed through partial amplification of the mitochondrial cytochrome C oxidase subunit I gene. Finally, positive samples were sequenced to determine the tapeworm species. Seven positive samples were obtained through polymerase chain reaction amplification. Sequencing confirmed that two samples were from T. solium and three samples were from Taenia hydatigena. The other two samples could not be specifically identified. Our findings demonstrate that dung beetles ingest T. solium and T. hydatigena eggs under natural conditions and suggest that beetles may play arole in the dynamics of transmission of these cestodes.
Start page
1198
End page
1200
Volume
99
Issue
5
Language
English
OCDE Knowledge area
Ciencia animal, Ciencia de productos lƔcteos
Scopus EID
2-s2.0-85056263609
PubMed ID
Source
American Journal of Tropical Medicine and Hygiene
ISSN of the container
0002-9637
Sources of information:
Directorio de Producción CientĆfica
Scopus