Title
Validation of five-colony pool analysis using multiplex real-time PCR for detection of diarrheagenic Escherichia coli
Date Issued
01 June 2009
Access level
open access
Resource Type
journal article
Author(s)
OCHOA WOODELL, THERESA JEAN
ECKER LEDESMA, LUCIE
GIL MERINO, ANA ISABEL
LANATA DE LAS CASAS, CLAUDIO FRANCO
Cleary T.
Abstract
Five Escherichia coli colonies/patient were studied to evaluate the reliability of a multiplex real-time PCR assay for detection of diarrheagenic Escherichia coli groups, using a pool of five colonies rather than individual colonies. Sensitivity and specificity were 98% and 100%, respectively, at a fifth of the cost of the individual colony analysis. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Start page
1915
End page
1917
Volume
47
Issue
6
Language
English
OCDE Knowledge area
BiologÃa celular, MicrobiologÃa
Ciencias médicas, Ciencias de la salud
Scopus EID
2-s2.0-66749151049
PubMed ID
Source
Journal of Clinical Microbiology
ISSN of the container
00951137
Sponsor(s)
Fogarty International Center - K01TW007405
Eunice Kennedy Shriver National Institute of Child Health and Human Development - R01HD051716
Sources of information:
Directorio de Producción CientÃfica
Scopus