Title
Validation of five-colony pool analysis using multiplex real-time PCR for detection of diarrheagenic Escherichia coli
Date Issued
01 June 2009
Access level
open access
Resource Type
journal article
Abstract
Five Escherichia coli colonies/patient were studied to evaluate the reliability of a multiplex real-time PCR assay for detection of diarrheagenic Escherichia coli groups, using a pool of five colonies rather than individual colonies. Sensitivity and specificity were 98% and 100%, respectively, at a fifth of the cost of the individual colony analysis. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Start page
1915
End page
1917
Volume
47
Issue
6
Language
English
OCDE Knowledge area
Biología celular, Microbiología Ciencias médicas, Ciencias de la salud
Scopus EID
2-s2.0-66749151049
PubMed ID
Source
Journal of Clinical Microbiology
ISSN of the container
00951137
Sponsor(s)
Fogarty International Center - K01TW007405 Eunice Kennedy Shriver National Institute of Child Health and Human Development - R01HD051716
Sources of information: Directorio de Producción Científica Scopus