Title
Serologic diagnosis of human Taenia solium cysticercosis by using recombinant and synthetic antigens in QuickELISA™
Date Issued
01 April 2011
Access level
open access
Resource Type
journal article
Author(s)
Lee Y.M.
Handali S.
Hancock K.
Pattabhi S.
Kovalenko V.A.
Levin A.
Lin S.
Scheel C.M.
Tsang V.C.W.
Abstract
Diagnosis of Taenia solium cysticercosis is an important component in the control and elimination of cysticercosis and taeniasis. New detection assays using recombinant and synthetic antigens originating from the lentil lectin-purified glycoproteins (LLGPs) of T. solium cysticerci were developed in a QuickELISA™ format. We analyzed a panel of 474 serum samples composed of 108 serum samples from donors with two or more viable cysts, 252 serum samples from persons with other parasitic infections, and 114 serum samples from persons with no documented illnesses. The sensitivities and specificities of T24H QuickELISA™, GP50 QuickELISA™, and Ts18var1 QuickELISA™ were 96.3% and 99.2%, 93.5% and 98.6%, and 89.8% and 96.4%, respectively, for detecting cases with multiple, viable cysts. T 24H QuickELISA™ performs best among the three assays, and has sensitivity and specificity values comparable to those of the LLGP enzyme-linked immunosorbent blot. The QuickELISA™ are simple, rapid quantitative methods for detecting antibodies specific for T. solium cysticerci antigens. Copyright © 2011 by The American Society of Tropical Medicine and Hygiene.
Start page
587
End page
593
Volume
84
Issue
4
Language
English
OCDE Knowledge area
Parasitología
Scopus EID
2-s2.0-79958013069
PubMed ID
Source
American Journal of Tropical Medicine and Hygiene
ISSN of the container
00029637
Sponsor(s)
National Institute of Allergy and Infectious Diseases R43AI064988
Sources of information:
Directorio de Producción Científica
Scopus